Premium
Kinetics of non‐photosynthetic callus production from the brown alga Laminaria setchellii (Laminariales)
Author(s) -
Qi Hans,
Rorrer Kristin A.,
Jiang Zhi D.,
Rorrer Gregory L.
Publication year - 1995
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/j.1440-1835.1995.tb00023.x
Subject(s) - callus , explant culture , laminaria , biology , botany , stipe (mycology) , photosynthesis , algae , biochemistry , in vitro
SUMMARY White, filamentous, callus‐like tissue was produced from the temperate marine brown alga Laminaria setchellii Silva under non‐photosynthetic conditions. Explant disks from the medullary core of the stipe section were plated on solid medium (1.5 wt% agar in seawater base, pH 8) at 8 ± 2°C in the dark, and the formation of callus‐like tissue was followed as a function of time up to 8 weeks. Although 30% of the total explants plated formed filamentous, callus‐like growths, only 3 to 6% of the total explants produced relatively large amounts of filamentous and rounded callus‐like cells. The fresh weight yield of this callus‐like tissue was 3.74 ± 0.93 mg per 10 mm explant disk (0.053 g callus per g explant). Attempts at establishing a growing, cell suspension of the dispersed callus tissue in PES liquid medium at 24 μmol photon m −2 s −1 were not successful.