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Studies on inoculation of aerial algae. I. Semi‐mass culture and quantification of algal color of Klebsormidium flaccidum (Ulotrichales, Chlorophyceae) sprayed on to concrete board
Author(s) -
Nanba Nobuyoshi,
Machii Minori,
Keng Lim Boon,
Sakurai Nobuo
Publication year - 1995
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/j.1440-1835.1995.tb00013.x
Subject(s) - biology , lightness , botany , algae , chromaticity , chlorophyceae , chlorophyta , aeration , horticulture , ecology , physics , optics
SUMMARY This paper describes methods of semi‐mass culture and the quantification of algal color sprayed on a concrete board of the alga Klebsormidium flaccidum (Küitzing) S. Mattox et Blackwell. The alga was cultured in 100 L tanks normally used for hatching brine shrimp, with aeration from the deepest parts of the slanting sides. Initially mean algal density increased rapidly and attained 9.1 times the original density after 4 weeks. Subsequently, increase of the algal density was slow, and the density became 16.1 times the original level after 8 weeks. The alga cultured in this study was sprayed on to a concrete board using an agricultural sprayer. The relationship between the algal density on the board and its color, expressed as L*a*b* color space, was quantified. The chromaticity was maximum as the algal density on the board was about 5 g/m 2 , and the color was most vivid. When the algal density was more than 20 g/m 2 , both the lightness and chromaticity changed slightly, and the color became dark green.