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DNA content of Ulva compressa (Ulvales, Chlorophyta) nuclei determined with laser scanning cytometry
Author(s) -
Kagami Yayoi,
Fujishita Mariko,
MatsuyamaSerisawa Kazuyo,
Yamamoto Maki,
Kuwano Kazuyoshi,
Saga Naotsune,
Kawano Shigeyuki
Publication year - 2005
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/j.1440-183.2005.00375.x
Subject(s) - biology , chlorophyta , botany , dna , algae , genetics
SUMMARY The green macroalgal genus Ulva (incl. Entemmorpha ) contains economically valuable species, is of relevance for coastal management (green tides), and certain taxa serve as experimental organisms for fundamental research in green algae. The nuclear genome size of Ulva (Entemmorpha) compressa Linnaeus was measured in propidium iodide stained nuclei using laser scanning cytometry. Nuclei of fixed gametes yielded reproducible values, whereas nuclei extracted from multicellular gametophytes were unsuitable. With nuclei of Arabidopsis thaliana (L.) Heynh and Saccharomyces cerevisiae Hansen as references, the haploid nuclear genome size of U. compressa was calculated as 135 ± 7 Mbp. This is the smallest genome so far known from any species of Ulva .