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Histone H1 expression in human prostate cancer tissues and cell lines
Author(s) -
Sato Shinya,
Takahashi Satoru,
Asamoto Makoto,
Nakanishi Makoto,
Wakita Toshiaki,
Ogura Yuji,
Yatabe Yasushi,
Shirai Tomoyuki
Publication year - 2012
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2011.02755.x
Subject(s) - lncap , histone , prostate cancer , histone h1 , cancer research , androgen receptor , biology , gene silencing , cancer , gene , genetics
Histone H1, one of the histone superfamilies, is known to determine chromatin structure and alter gene expression. It also contributes to regulation of cell proliferation in breast cancer. We hypothesized a similar association in prostate cancer, and therefore examined relationships between histone H1 expression and Gleason pattern, Ki‐67 and androgen receptor levels in a series of prostate cancer tissues and cell lines. Histone H1 positive cancer cells increased with the Gleason pattern. Gleason pattern 3 tumors were divided into two groups, one with high histone H1 positivity (H1‐high cases, 60–100% positivity) and the other with low histone H1 positivity (H1‐low cases, 0–20% positivity). Ki‐67 or androgen receptor positivity in H1‐high cases was significantly higher than in H1‐low cases. PC3 cells demonstrated more frequent histone H1 and Ki‐67 positivity as compared to LNCaP cells. Silencing of histone H1 by siRNA transfection significantly reduced cell proliferation in LNCaP and PC3. These findings suggest that histone H1 expression is associated with the Gleason pattern, cell proliferation and androgen receptor expression in prostate cancers.