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Fluorescence in situ hybridization analysis with a tissue microarray: ‘FISH and chips’ analysis of pathology archives
Author(s) -
Sugimura Haruhiko,
Mori Hiroki,
Nagura Kiyoko,
Kiyose Shinichiro,
Hong Tao,
Isozaki Masaru,
Igarashi Hisaki,
Shinmura Kazuya,
Hasegawa Akio,
Kitayama Yasuhiko,
Tanioka Fumihiko
Publication year - 2010
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2010.02561.x
Subject(s) - fluorescence in situ hybridization , tissue microarray , comparative genomic hybridization , biology , pathology , somatic cell , in situ hybridization , computational biology , microarray , chromosome , cancer , genetics , medicine , gene , gene expression
Practicing pathologists expect major somatic genetic changes in cancers, because the morphological deviations in the cancers they diagnose are so great that the somatic genetic changes to direct these phenotypes of tumors are supposed to be correspondingly tremendous. Several lines of evidence, especially lines generated by high‐throughput genomic sequencing and genome‐wide analyses of cancer DNAs are verifying their preoccupations. This article reviews a comprehensive morphological approach to pathology archives that consists of fluorescence in situ hybridization with bacterial artificial chromosome (BAC) probes and screening with tissue microarrays to detect structural changes in chromosomes (copy number alterations and rearrangements) in specimens of human solid tumors. The potential of this approach in the attempt to provide individually tailored medical practice, especially in terms of cancer therapy, is discussed.