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Comparison of immunohistochemistry assays and real‐time reverse transcription–polymerase chain reaction for analyzing hormone receptor status in human breast carcinoma
Author(s) -
Oda Miyo,
Arihiro Koji,
Kataoka Tsuyoshi,
Osaki Akihiko,
Asahara Toshimasa,
Ohdan Hideki
Publication year - 2010
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2010.02522.x
Subject(s) - immunohistochemistry , progesterone receptor , concordance , reverse transcription polymerase chain reaction , breast cancer , estrogen receptor , hormone receptor , polymerase chain reaction , real time polymerase chain reaction , medicine , estrogen , pathology , breast carcinoma , biology , cancer , messenger rna , gene , biochemistry
The estrogen receptor (ER) and progesterone receptor (PgR) status of 163 surgical breast cancer specimens determined on real‐time quantitative reverse transcription–polymerase chain reaction (RT‐PCR) using frozen tumor tissue were compared with that determined using three automated immunohistochemistry (IHC) assays including Dako (Glostrup, Denmark), Ventana (Tucson, AZ, USA) and BioGenex (San Ramon, CA, USA) assay. All specimens were semiquantified according to the Allred score and J‐score. The cut‐offs for ER determined by log (ER/glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH)) were −3.6 and −3.2 based on the Allred score and J‐score, respectively, and those for PgR determined by log (PgR/GAPDH) were −3.2 and −2.8, respectively. The Allred total score (TS) and the J‐score for ER and PgR on IHC were significantly correlated with the result on RT‐PCR ( P < 0.00001). There was a high degree of concordance among ER and PgR status on IHC and those on RT‐PCR, suggesting that RT‐PCR is a useful method for evaluation of ER and PgR status. Some discrepancies between the IHC and RT‐PCR results were identified, however. Accordingly, further studies of RT‐PCR assays for hormone receptor (HR) are necessary with regard to biological behavior and responsiveness to hormone therapy.