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Expression of USP2‐69 in mesangial cells in vivo and in vitro
Author(s) -
Wang Suxia,
Wu Huijuan,
Liu Ye,
Sun Jianyong,
Zhao Zhonghua,
Chen Qi,
Guo Muyi,
Ma Duan,
Zhang Zhigang
Publication year - 2010
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2010.02496.x
Subject(s) - mesangial proliferative glomerulonephritis , proliferating cell nuclear antigen , lupus nephritis , cell growth , mesangial cell , pathology , immunohistochemistry , kidney , biology , medicine , chemistry , glomerulonephritis , endocrinology , disease , genetics
Ubiquitin‐specific protease 2 (USP2) is a member of a family of de‐ubiquitinating enzymes. It may play an important role in the regulation of cell growth and differentiation. It is known that expression of the isoform USP2‐69 kD is high in kidney tissue, but its role remains unclear. Mesangial cell proliferation is a prominent element of various types of glomerulonephritides. Therefore, whether USP2 plays a role in mesangial cell proliferation during glomerulonephritides is an interesting question to explore. The purpose of the present study was to evaluate USP2‐69 expression in needle biopsies of human kidneys and in cultured rat mesangial cells. On immunohistochemistry USP2‐69 was upregulated in some mesangial proliferative glomerulonephritides. The proportion of USP2‐69 positive area in the glomeruli was 3.90% in normal kidney, 4.96% in minimal change disease, and 4.39% in membranous glomerulonephritides, while it was 14.84% in IgA nephropathy (IgAN) (mesangial proliferative type), 16.18% in lupus nephritis (LN; diffuse proliferative type) and 15.54% in acute proliferative glomerulonephritides (APGN); the difference of the percentages between IgAN, LN (IV subtype) and APGN and normal kidney were statistically significant ( P < 0.05). Additionally, the number of proliferating cell nuclear antigen (PCNA)‐positive nuclei in the glomeruli was statistically significantly higher in the various glomerulonephritides than in the normal kidney ( P < 0.05). Immunohistochemistry showed that the distribution of the USP2 + area and PCNA + nuclei overlapped in the glomeruli. Treatment with interleukin‐1β for 12 h and 24 h, or with anti‐thymocyte serum for 6 h and 12 h resulted in elevated USP2‐69 mRNA and protein expression in the rat mesangial cells. Also, PCNA expression increased and p27 expression decreased significantly in the treated mesangial cells. These findings suggest that USP2‐69 was upregulated in mesangial cells during mesangial proliferative glomerulonephritides in vivo and in vitro , which may relate to the proliferation of mesangial cells.