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Quantitative assessment of gene methylation in neoplastic and non‐neoplastic gastric epithelia using methylation‐specific DNA microarray
Author(s) -
Tamura Gen,
So Kanji,
Miyoshi Hiroaki,
Honda Teiichiro,
Nishizuka Satoshi,
Motoyama Teiichi
Publication year - 2009
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2009.02458.x
Subject(s) - methylation , dna methylation , microbiology and biotechnology , biology , cancer , cpg site , cancer research , gene , gene expression , genetics
A fiber‐type DNA microarray was used to calculate methylation rates (MR) of four tumor suppressor genes, lysyl oxidase ( LOX ), p16 , RUNX3 , and tazarotene‐induced gene 1 ( TIG1 ). MR were calculated in 26 primary gastric cancers and corresponding non‐neoplastic gastric epithelia, and the results were compared to those of conventional methylation‐specific polymerase chain reaction (MSP). MR ranged from 0.1% to 69.1% (mean, 18.3%) for LOX , 0.5–74.1% (mean, 15.7%) for p16 , 0.2–76.5% (mean, 22.7%) for RUNX3 , and 0.6–41.2% (mean, 5.8%) for TIG1 in primary gastric cancers, and from 0.1% to 25.8% (mean, 8.7%) for LOX , 1.0– 23.2% (mean, 10.3%) for p16 , 0.7–25.1% (mean, 5.5%) for RUNX3 , and 1.8–27.6% (mean, 11.4%) for TIG1 in corresponding non‐neoplastic gastric epithelia. Although MR varied significantly across different samples for both neoplastic and non‐neoplastic gastric epithelia, high‐level methylation (MR >40%) was cancer specific and was observed in 19.2%, 19.2%, 30.8%, and 3.8% of primary gastric cancers for LOX , p16 , RUNX3 , and TIG1 , respectively. All samples with high‐level methylation, as well as some samples with low MR (particularly <10%) were judged to be methylation positive on conventional MSP. Quantitative analysis of gene methylation using methylation‐specific DNA microarray is a promising method for cancer diagnosis.