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Immunohistochemical staining for P1 and P2 promoter‐driven hepatocyte nuclear factor‐4α may complement mucin phenotype of differentiated‐type early gastric carcinoma
Author(s) -
Takano Kabuto,
Hasegawa Go,
Jiang Shuying,
Kurosaki Isao,
Hatakeyama Katsuyoshi,
Iwanari Hiroko,
Tanaka Toshiya,
Hamakubo Takao,
Kodama Tatsuhiko,
Naito Makoto
Publication year - 2009
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2009.02394.x
Subject(s) - pathology , mucin , immunohistochemistry , foveolar cell , phenotype , biology , stomach , gastric mucosa , medicine , gene , biochemistry
Hepatocyte nuclear factor 4α (HNF4α) isoforms in the human stomach have not been fully investigated. The purpose of the present study was to evaluate the expression of P1 and P2 promoter‐driven HNF4α (P1 and P2‐HNF4α) in differentiated‐type early gastric carcinomas (DEGC). P1‐ and P2‐HNF4α expression was examined immunohistochemically both in non‐neoplastic mucosa and carcinoma from surgical specimens. In all samples of non‐neoplastic mucosa, foveolar, cardiac, fundic and pyloric gland epithelium was negative for P1‐HNF4α, but was positive for P2‐HNF4α. Intestinal metaplasia was positive for P1 and P2‐HNF4α in all cases. Gastric carcinomas were classified into four mucin phenotypes based on the pattern of mucin expression: gastric, intestinal, mixed and null type. DEGC showed striking differences in the staining pattern for P1‐HNF4α according to the mucin phenotype. Gastric carcinomas of intestinal, mixed and null type showed high positivity for P1‐HNF4α, but the gastric type was negative for P1‐HNF4α in all but one tumor. In contrast, P2‐HNF4α was expressed in all tumors regardless of the mucin phenotype. Negative expression of P1‐HNF4α was indicated as one of the useful immunohistochemical markers in the classification of mucin phenotype of both non‐neoplastic mucosa and cancers of gastric phenotype.