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Detection of HPV‐DNA, p53 alterations, and methylation in penile squamous cell carcinoma in Japanese men
Author(s) -
Yanagawa Naoki,
Osakabe Mitsumasa,
Hayashi Masahiro,
Tamura Gen,
Motoyama Teiichi
Publication year - 2008
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2008.02259.x
Subject(s) - methylation , dna methylation , gene , fhit , penile carcinoma , cancer research , carcinoma , biology , pathology , penile cancer , tumor suppressor gene , medicine , cancer , gene expression , carcinogenesis , genetics
Penile carcinoma is a rare disease, accordingly there are few studies on molecular changes, and these results also vary greatly. A total of 26 penile squamous cell carcinomas in Japanese men were studied with respect to HPV, p53 alterations, and methylation of gene promoter region. HPV‐DNA was detected in three of 26 patients (11.5%). Overexpression of p53 was observed in 13 of 26 patients (50%), and p53 gene mutations were detected in four of 26 patients (15.4%). The frequency of methylation was as follows: DAPK , 26.9% (7/26); FHIT , 88.4% (23/26); MGMT , 19.2% (5/26); p14 , 3.8% (1/26); p16 , 23.1% (6/26); RAR ‐β, 23.1% (6/26); RASSF1A , 11.5% (3/26); and RUNX3 , 42.3% (11/26). As for correlation between HPV and p53 alterations, and methylation status, mutations of the p53 gene were detected only in HPV‐negative patients, and methylation was more frequently found in HPV‐negative than in HPV‐positive patients. The present results suggest that the majority of penile squamous cell carcinomas in Japanese men are unrelated to HPV, and gene alterations accumulate more frequently in HPV‐unrelated penile carcinomas.