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Downregulation of Krüppel‐like factor 9 in human colorectal cancer
Author(s) -
Kang Ling,
Lü Bingjian,
Xu Jing,
Hu Hu,
Lai Maode
Publication year - 2008
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2008.02233.x
Subject(s) - krüppel , colorectal cancer , angiogenesis , reverse transcription polymerase chain reaction , messenger rna , carcinogenesis , immunohistochemistry , downregulation and upregulation , cancer research , cancer , zinc finger , biology , western blot , medicine , pathology , microbiology and biotechnology , gene expression , transcription factor , gene , genetics
Mammalian Sp and Krüppel‐like factors (KLF), a family of zinc finger‐containing transcription factors, are involved in growth control, proliferation, apoptosis and angiogenesis of a wide variety of tissues and cells. Several KLF have been linked to various types of human cancers, but the relationship between Krüppel‐like factor 9 (KLF9) and colorectal cancer has not been explored. The purpose of the present study was to investigate KLF9 expression in human colorectal cancer tissue. KLF9 mRNA was detected on quantitative real‐time reverse transcriptase–polymerase chain reaction (Q‐PCR). Of the 50 cancerous tissues examined, 86% (43/50) expressed lower levels of KLF9 mRNA than individually matched normal mucosa ( P < 0.0001). On western blot, reduced or absent expression of KLF9 protein was observed in 65% (13/20) of the samples ( P < 0.01). A total of 81% (35/43) of normal samples had expression of KLF9 protein, whereas its protein was detected in only 9% (4/43) of tumor tissues ( P < 0.001) on tissue microarray. These results indicate that KLF9 may be involved in the carcinogenesis of human colorectal cancer.