Premium
Expression of ADAMTS4 (aggrecanase‐1) in human osteoarthritic cartilage
Author(s) -
Naito Satoko,
Shiomi Takayuki,
Okada Aiko,
Kimura Tokuhiro,
Chijiiwa Miyuki,
Fujita Yoshinari,
Yatabe Taku,
Komiya Koichiro,
Enomoto Hiroyuki,
Fujikawa Kyosuke,
Okada Yasunori
Publication year - 2007
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2007.02167.x
Subject(s) - adamts , aggrecan , aggrecanase , cartilage , proteoglycan , chondrocyte , thrombospondin , metalloproteinase , chemistry , type ii collagen , osteoarthritis , pathology , matrix metalloproteinase , anatomy , microbiology and biotechnology , articular cartilage , medicine , biology , biochemistry , alternative medicine
A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)1, 4, 5, 8, 9 and 15, members of the ADAMTS gene family, have the ability to degrade a major cartilage proteoglycan, aggrecan, at the specific sites, and thus are called ‘aggrecanases’. The expression of these ADAMTS species was examined in human osteoarthritic articular cartilage on reverse transcription–polymerase chain reaction. The results demonstrated the predominant expression of ADAMTS4 in osteoarthritic cartilage, while ADAMTS5 was constitutively expressed in osteoarthritic and normal cartilage. ADAMTS9 was expressed mainly in normal cartilage, whereas no or negligible expression of ADAMTS1 , 8 and 15 was observed in either osteoarthritic or normal cartilage. In situ hybridization for ADAMTS4 indicated that chondrocytes in osteoarthritic cartilage expressed the mRNA. Two monoclonal antibodies to ADAMTS4 were developed, and immunolocalized ADAMTS4 to chondrocytes in the proteoglycan‐depleted zones of osteoarthritic cartilage, showing a direct correlation with the Mankin scores. Immunoblotting indicated a major protein band of 58 kDa in the chondrocyte culture media and osteoarthritic cartilage tissue homogenates. These data demonstrate that among the six ADAMTS species, ADAMTS4 is mainly expressed in an active form in osteoarthritic cartilage, and suggest that ADAMTS4 may play an important role in the degradation of aggrecan in human osteoarthritic cartilage.