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Repeated fluorescence in situ hybridization by a microwave‐enhanced protocol
Author(s) -
Kitayama Yasuhiko,
Igarashi Hisaki,
Kozu Taeko,
Nagura Kiyoko,
Ohashi Yohei,
Sugimura Haruhiko
Publication year - 2006
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2006.01996.x
Subject(s) - in situ , fluorescence in situ hybridization , microwave , fluorescence , in situ hybridization , pathology , protocol (science) , medicine , computational biology , chemistry , computer science , biology , physics , optics , biochemistry , telecommunications , organic chemistry , chromosome , gene , gene expression , alternative medicine
A novel re‐hybridization protocol for pathology archive sections that uses microwave‐assisted fluorescence in situ hybridization (FISH) is described. Stripping the probe from the pathology archive sections with HCl and re‐hybridizing with the next probe by intermittent microwave irradiation generated clear signals without background noise. Repeated stripping and hybridization with numerous bacterial artificial chromosome (BAC)‐derived probes would identify the profile of genome‐wide changes in small lesions on sections.