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Serum‐dependence of AMPA receptor‐mediated proliferation in glioma cells
Author(s) -
Yoshida Yukari,
Tsuzuki Keisuke,
Ishiuchi Shogo,
Ozawa Seiji
Publication year - 2006
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2006.01954.x
Subject(s) - ampa receptor , glutamate receptor , receptor , fetal bovine serum , cell growth , microbiology and biotechnology , cell culture , glioma , cell , chemistry , biology , cancer research , biochemistry , genetics
Glutamate may cause Ca 2+ entry through Ca 2+ ‐permeable glutamate receptors, which in turn stimulates the anti‐apoptotic signaling cascade in glioma cells. It was found that a human glioma cell line, U‐87 MG, expressed subunits of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionate acid‐type glutamate receptors (AMPAR). Ca 2+ entry through AMPAR was detected in approximately 40% of U‐87 MG cells. AMPAR agonists facilitated cell proliferation in low‐serum medium containing 0.5% fetal calf serum (FCS). Unexpectedly, cell proliferation by the activation of AMPAR was not detected in serum‐rich medium containing 10% FCS. Overexpression of Ca 2+ ‐permeable AMPAR facilitated proliferation of U‐87 MG cells in the low‐serum condition, whereas it had again no effect in the serum‐rich condition. Cell proliferation of U‐87 MG cells is likely to be under the regulation of both growth factors contained in the serum and Ca 2+ entry through AMPAR, and that the latter regulation becomes evident only when serum factors are deprived of culture medium.