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Establishment and characterization of new cell lines derived from melanotic neuroectodermal tumor of infancy arising in the mandible
Author(s) -
Metwaly Hamdy,
Cheng Jun,
Maruyama Satoshi,
Ohshiro Kazufumi,
Suzuki Ichiro,
Hoshina Yukiko,
Saku Takashi
Publication year - 2005
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2005.01833.x
Subject(s) - biology , pathology , glial fibrillary acidic protein , vimentin , myelin basic protein , neuroectodermal tumor , carcinoembryonic antigen , synaptophysin , neurofilament , microbiology and biotechnology , immunohistochemistry , genetics , immunology , myelin , medicine , central nervous system , cancer , neuroscience
Three cell systems (MINT1/2/3) derived from a melanotic neuroectodermal tumor of infancy (MNTI) arising in the mandible of a 1‐month‐old newborn boy have been established, and their cytological natures have been characterized. The cells had immunopositivities for pan‐keratin, vimentin, neuron‐specific enolase, S‐100 protein and melanoma‐associated antigen (HMB‐45). These immunohistochemical phenotypes were basically the same as those observed in tissue sections, in which, synaptophysin, myelin basic protein, c‐myc gene products, carcinoembryonic antigen, and epithelial membrane antigen were also immunolocalized in tumor cells. Karyotyping analyzes revealed that the chromosome numbers of the three cell systems ranged from 60 to 67 with 3n ploidies, and that there were many structural aberrations, such as del(11)(q13), del(22)(q13), add(2)(p11), add(7)(q22), extra copies for chromosomes 1, 2, 3, 5, 7, 9, 10, 11, 12, 16, 20, and 22, der(9)t(9;13)(p13;q12)add(9)(q34), and der(13;21)(q10;q10), which were shared by the three cell systems, while der(19)t(11;19)(q13;p13) was found in MINT1 and MINT3. When stimulated by endothelin‐3 and vitamin D 3 , the cells had spinous cell shapes with immunopositivities for HMB‐45, neurofilament protein and glial fibrillary acidic protein, which indicated more neural differentiation. The established cell systems will be useful for further investigation on the molecular and genetic basis of MNTI to understand its pathogenesis, which is largely unknown.