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Immunohistochemical analysis of cleaved caspase‐3 detects high level of apoptosis frequently in diffuse large B‐cell lymphomas of the central nervous system
Author(s) -
Arai Momoko,
Sasaki Atsushi,
Saito Nobuhito,
Nakazato Yoichi
Publication year - 2005
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2005.01808.x
Subject(s) - immunohistochemistry , tunel assay , apoptosis , pathology , lymphoma , caspase 3 , diffuse large b cell lymphoma , biology , terminal deoxynucleotidyl transferase , central nervous system , cancer research , programmed cell death , medicine , endocrinology , biochemistry
The purpose of the present paper was to examine the level of apoptosis and the relationships among apoptosis, apoptosis‐associated proteins, and proliferating potential in lymphoma tissues to clarify the characteristics of apoptosis in diffuse large B‐cell lymphomas (DLBCL) of the central nervous system (CNS). The formalin‐fixed, paraffin‐embedded tissues of CNS and non‐CNS DLBCL (20 cases each) were studied by terminal deoxynucleotidyl transferase‐mediated dUTP‐nick end labeling (TUNEL) and immunohistochemistry, using antibodies against single‐stranded DNA (ssDNA), cleaved caspase‐3, bcl‐2, bax, p53, Fas and Ki‐67. The cleaved caspase‐3 immunohistochemistry detected apoptosis of the lymphoma cells most sensitively compared to TUNEL and ssDNA immunohistochemistry. High expression (grade + + or + + +) of cleaved caspase‐3 was found more frequently in CNS DLBCL (11 cases, 55%) than non‐CNS DLBCL (three cases, 15%; P = 0.009). Bax‐positivity of lymphoma cells was increased in six cases of CNS DLBCL, which also showed high positivity of cleaved caspase‐3. There was no significant correlation between the cleaved caspase‐3‐positivity and the Ki‐67 positivity. The present study indicates that the number of apoptotic cells and expression level of cleaved caspase‐3 were significantly higher in CNS DLBCL than non‐CNS DLBCL, and that the correlation of bax and cleaved caspase‐3 expression was often present in CNS DLBCL.