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Immunohistochemical characterization of two novel monoclonal antibodies that recognize human perivascular cells of the central nervous system and macrophage subsets
Author(s) -
Yokoo Hideaki,
Sasaki Atsushi,
Hirato Junko,
Nakazato Yoichi
Publication year - 1998
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1998.tb03968.x
Subject(s) - macrophage , monoclonal antibody , pathology , biology , immunohistochemistry , monocyte , antibody , antigen , red pulp , staining , microbiology and biotechnology , immunology , spleen , medicine , in vitro , biochemistry
The monoclonal antibodies to cells of monocyte/macraphege lineage were established using a human glial cell‐rich fraction as the Immunogen. The antibodies, named GP‐1 and GP‐2, were originally found to react with perivascular cells of the central nervous system. They are Immuno‐hiaochemically applicable on routinely formalin‐fixed, paraffin‐embedded tissue sections. GP‐1 binds to a lysosomal protein, and GP‐2 to a carbohydrate epitope of the cell membrane and lysosomes. Among the visceral organs, GP‐1 labeled blood monocytes, almost ail kinds of tissue and Infiltrating macrophages in both normal and diseased state, and renal tubules. GP‐1 staining of tissue macrophages tends to be Intensified under Inflammatory conditions. GP‐1 staining also suggested that perivascular cells and macrophages had different ontogeny. GP‐2 immunostained monocytes, Kupffer's cells, red pulp macrophages, Infiltrating macrophages and reactive microglla, but not alveolar or tingible body macrophages. Besides those macrophages, GP‐2 stained mantle zone lymphocytes, some hematopoietic cells, pneumocytes and renal collecting ducts. The staining pattern of ligands on THP‐1 and HL40 neoplastic human macrophage cell lines was dissimilar to that of other macrophage markers, suggesting that they recognize unknown macrophage‐related antigens.