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Immunohistochemical overexpression of p16 protein associated with intact retinoblastoma protein expression in cervical cancer and cervical intraepithelial neoplasia
Author(s) -
Sano Takaaki,
Oyama Tetsunarl,
Kashiwabara Kenji,
Fukuda Toshio,
Nakajima Takashi
Publication year - 1998
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1998.tb03954.x
Subject(s) - immunohistochemistry , retinoblastoma , cervical cancer , carcinogenesis , cervical intraepithelial neoplasia , retinoblastoma protein , pathology , biology , cancer , intraepithelial neoplasia , cancer research , medicine , cell cycle , gene , prostate , biochemistry
Both p16 and retinoblastoma (Rb) proteins are important tumor supprsssors that regulate the cell cycle. The status of both proteins In lnvasive cervical cancer and cervical intraepithelias neopiasis (CIN) has not yet been examined. The aim of this study was to investigate the expression of p16 and Rb proteins by immunohlstochemistry using 98 formalln‐fixed and paraffin‐embedded samples of various cervical neoplastic lesions. Strong immunoreactiyity for the p16 protein was observed in both the nuclei and cytoplasm of all CIN and lnvasive cancer cases except several low‐grade CIN lesions. Expression of Rb protein was also demonatrated In the scattered nuclel of neoplastic and normal cells in all cases Investigated. The results suggest that the deletion or mutational inactivity of both p16 and Rb proteins may be a rare event In cervical carcinogenesls. Moreover, overexpression of the p16 protein may be a useful dlagnostic marker for cervical neoplastic lesions on routine laboratory screening.