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An immunohistochemical study of amyloid P component, apolipoprotein E and ubiquitin in human and murine amyloidoses
Author(s) -
Cul Dan,
Hoshll Yoshinobu,
Takahashl Mutsuo,
Kawano Hiroo,
Iwata Takako,
Ishihara Tokuhiro
Publication year - 1998
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1998.tb03919.x
Subject(s) - amyloid (mycology) , spleen , pathology , immunohistochemistry , amyloidosis , apolipoprotein b , chemistry , serum amyloid p component , biology , biochemistry , medicine , immunology , inflammation , c reactive protein , cholesterol
Amyloid P component (AP) and apolipoprotein E (Apo E), which are known to be minor constituents of amyloid deposits, commonly are associated with almost all types of amyloid deposits. In this study, the distribution of AP‐, Apo E‐ and ubiquitin (Ub)‐lmmunoreactlvlty (IR) In amyloid deposits In the liver and spleen of human systemic amyloldosls (34 autopsy cases: 17 Immunoglobulln light chain derived, 17 amyloid A protein derived) and experimental murine amyloldosls is examined using an immunohistochemical technique. In human cases, all of the amyloid deposits examined showed colocallzation of AP‐ and Apo E‐IR with Individual amyloid proteins. In experimental amyloldosls, AP‐IR of amyloid deposits in the liver and spleen and Apo E‐IR in the liver were seen uniformly throughout this experiment. In contrast, Apo E‐IR In the spleen was not uniform at the phase of amyloid deposition. At 4 weeks and at 16 weeks after casein injection, Apo E‐IR was unevenly distributed in amyloid deposits in the perifollicular area; however, from 6 to 12 weeks it was seen to be uniform. Ublqultin‐IR of amyloid deposits in human cases was seen In 22 of 34 livers and in 22 of 33 spleens. In experimental amyloldosls, Ub‐IR of amyloid deposits was demonstrated In the space of Disse In all mice examined, and there appeared to be a gradual Increase in intensity with the amount of amyloid deposition. However, in the spleen, amyloid deposits did not react with anti‐Ub antibody In any phase of amyloid Induction. These results suggest that Apo E and Ub are not always associated with the process of amyloid deposition and may appear in a deposit after the deposition.