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Cytofluorometric DNA analysis by stathmokinetic method of experimental thyroid neoplasms in rats
Author(s) -
Imanaka Masashi,
Yamamoto Yuzo,
Okatoh Fumiyuki,
Itoh Takashi,
Takahashi Hiroaki
Publication year - 1998
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1998.tb03878.x
Subject(s) - pathology , thyroid , thyroid tumors , dna , medicine , biology , endocrinology , thyroid carcinoma , genetics
Thyroid neoplasms were Induced In rats by the intraperitoneal Injection of N ‐bis(2‐hydroxy‐propyl)nltrosamlne as a carcinogenic substance and by the oral administration of KCIO 4 as a promoter. The nuclear DNA content was measured in tissue sections of the tumors by the stathmokinetic method with the use of vincristine, and was correlated with the hlstopathological findings In the process of tumorigenesls and progression. Hlstological examination showed various types of lesions were produced In 47 surviving rats. A diplold pattern was seen In 82 (92%) of 89 tumors and an aneuplold pattern in 7 (8%) of 89 tumors. Papillary carcinomas and mixed carcinomas only had a dlploid pattern, while two of 17 follicular carcinomas and five of 13 anaplastic carcinomas had an aneuploid pattern. On the other hand, benign proliferative nodules only had a dlploid pattern. DNA heterogeneity was noted in seven malignant tumors. In four of them it was associated with the histo‐pathological evidence of change, but in three there was no histologlcal difference between the aneuplofd and diploid areas other than a difference in the mitotic index. Mitotic indices were significantly higher in the aneuploid than in the diploid areas. These findings suggest that changes in the DNA ploidy cause histologlcal abnormalities and/or affect the mitotic index, ft is hypothesized that a change occurs in some diploid ceils in a region of a tumor, and that aneuploid cells develop from the diploid cells which then acquire high proliferatlve activity.