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Immunohistochemical and ultrastructural studies of basal cells, Clara cells and bronchiolar cuboidal cells in normal human airways
Author(s) -
Nakajima Yichiko,
Kawanami Oichi,
Jin Enjing,
Ghazizadeh Mohammad,
Honda Mitsuyoshi,
Asano Goro,
Horiba Koji,
Ferrans Victor J.
Publication year - 1998
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1998.tb03865.x
Subject(s) - hemidesmosome , cuboidal cell , anchoring fibrils , pathology , basal (medicine) , basement membrane , ultrastructure , intermediate filament , cell type , basal lamina , biology , chemistry , cell , immunohistochemistry , microbiology and biotechnology , anatomy , cytoskeleton , medicine , biochemistry , endocrinology , insulin
Immunohlstochemical studies were made of the distribution of various cytokeratins (CK), Clara cell secretory protein (CC10), surfactant proteln A (SP‐A) and type VII collagen in normal human alrways. Electron microscopic studies were made to Identify hemldesmosomes and anchoring fibrils on the basal surfaces of the epithelial cells. CK19 was detected In all epithelial cells, and CK17 in all basal cells. CK14 was coaxpressed in a few basal cells, and this coexpression was decreased in the distal airways. Two types of basal cells were recognized. One type, found mainly In large airways, was characterized by abundant intermediate filaments and well‐developed hemidesmosomes and anchoring fibrils. The second type contained few intermediate filaments and poorly developed hemidesmosomes and anchoring fibrils. Reactivity for type VII collagen was found along the basement membrane throughout the airways, but not in the alveoli. Clara cells were reactive for CC10 and CK17, but not for CK14 and SP‐A. The bronchiolar cuboidal cells in the respiratory bronchloles were positive only for CK19. Surfactant proteln A was present only in type II alveolar eplthellal cells. Thus, two types of basal cells are present in always, and the bronchiolar cuboidal cells appear distinct from these basal cells, Clara cells and type II alveolar epithelial cells.