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nm23‐H1 protein immunoreactivity in intraepithelial neoplasia and invasive squamous cell carcinoma of the uterine cervix
Author(s) -
Lee C. Soon,
Gad Jacqueline
Publication year - 1998
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1998.tb03841.x
Subject(s) - pathology , uterine cervix , basal cell , cervix , intraepithelial neoplasia , cervical intraepithelial neoplasia , medicine , carcinoma , cancer , cervical cancer , prostate
Differences In the Immunohlstochemlcal expression of the 17 kDa protein encoded by the human nm23‐H1 gene were studied In premallgnant lesions and Invasive squamous cell carcinoma (SCC) ( N = 8) of the cervix using routine streptavldln‐blotln Immunohistochemlstry and a polyclonal antibody to the nm23‐H1 protein. The premalignant lesions were kollocytic atypla due to wart virus Infection ( N = 5), low‐grade cervical intraepithelial neoplasia (CIN) ( N = 7) and high‐grade CIN ( N = 7). The carcinomas were either moderately ( N = 3) or poorly differentiated ( N = 5). The non‐neoplastlc controls were normal squamous epithelium from cases with uterine prolapse ( N = 7) and normal squamous epithelium not affected by the Infective or neoplastic areas of some of the cases with wart virus Infection ( N = 2) and carcinoma ( N = 2). Moderate to strong cytoplasmic and, occasionally, nuclear Immunostainlng for the nm23‐H1 protein was seen in all cells above the basal layer of the normal squamous epithelium. However, most of the cervical SCC show a relative reduction in nm23‐H1 immunoreactivity (7/8 cases; 88%). This difference In nm23‐H1 expression was statistically significant ( P = 0.0006; Chi‐squared test with continuity correction). All of the cases with wart virus Infection ( N = 5; 100%) displayed moderately strong nm23‐H1 immunostaining throughout the squamous epithelium except for the basal layer where no staining was observed. The cases that had low‐grade squamous dysplasia of the cervix (CIN Ml) ( N = 7; 100%) also displayed moderate to strong nm23‐H1 Immunoreactivity In the epithelium except for the basal layer (CIN I) or the lower two‐thirds of the epithelium (CIN II). nm23‐H1 Immunoreactivity was either absent or was significantly reduced in all of the high‐grade CIN (CIN III) cases (At = 7; 100%) in which only the non‐dysplastJc superficial third of the squamous epithelium displayed nm23‐H1 immunolabeling. The difference in nm23‐H1 expression between low‐grade and high‐grade CIN cases was statistically significant ( P = 0.0013; Chi‐squared test with continuity correction). Similarly, the difference between low‐grade CIN and SCC cases in the expression of nm23‐H1 was also significant ( P = 0.0041; Chi‐squared test with continuity correction). However, no statistically significant difference in nm23‐H1 immunoreactivity was found between cases of high‐grade CIN and SCC. In conclusion, nm23‐H1 protein immunoreactivity is reduced in high‐grade CIN and cervical SCC but not in low‐grade CIN. These findings suggest that reduced expression of the protein may be Important early in the sequential development of cervical squamous neoplasia.