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Expression of MUG1 glycoprotein in plasma cells, follicular dendritic cells, myofibroblasts and perineurial cells: lmmunohistochemical analysis using three monoclonal antibodies
Author(s) -
Kamoshida Shingo,
Tsutsumi Yutaka
Publication year - 1998
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1998.tb03837.x
Subject(s) - monoclonal antibody , myofibroblast , follicular dendritic cells , pathology , antibody , glycoprotein , immunohistochemistry , microbiology and biotechnology , biology , chemistry , medicine , immunology , antigen presenting cell , t cell , immune system , fibrosis
Normal and mallgnant plasma cells (PC), follicular dendritic cells (FDC), myoflbroblasts (MFB) and perineurial cells (PNC) were Investigated for the expression of YUC‐1 glycoprotein (MUC‐1 gp) by immunohistochemical and immunoelectron microscopic techniques uslng monocional antibodies E29, 115D8, DF3 and a combination of the three. MUG1 glycoprotein‐positive PC detected by the combined antibodies were frequently seen in a variety of pathological lesions tested, including chronic cervicitls, chronic syno‐vitis, Hodgkin's disease, allergic rhlnitis and sinusitis, tuberculous lymphadenitis, foreign body granuloma, multiple myeloma, and chronic tonsillitis. In the lesions containing MUC‐1gp‐positive PC, the infiltration of immunoglobulin (lg) E PC and/or IgE‐bound mast cells was significantly increased, but MUC‐1gp‐positive PC did not contain any specific immunoglobulin heavy or light chains. The findings suggest that the expression of MUC‐1gp In PC, although not restrlcted to IgE‐class cells, may be Induced in an allergic status. Plasma cells and PNC mainly reacted with the antibodies E29 and 115D8, while FDC and MFB were principally reactive wlth the antibody DF3. In some cases of multiple myeloma, the neoplastic PC were predominantly immunoreactive with DF3. The results indicate: (i) the epitopic variability of MUC1gp molecules expressed on the non‐epithelial cells; and (ii) the epitoplc alterations during malignant transformation. It should also be noted that the expression of MUG‐1 gp in the nonepithellal cells represents a pitfall in histopathological diagnosis.