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Detection of the Epstein‐Barr virus genome in cervical neoplasia is closely related to the degree of infiltrating lymphoid cells: A polymerase chain reaction and in situ hybridization approach
Author(s) -
Shojl Yoko,
Saegusa Makoto,
Takano Yasuo,
Hashimura Miki,
Okayasu Isao
Publication year - 1997
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1997.tb04532.x
Subject(s) - polymerase chain reaction , pathology , epstein–barr virus , virus , stroma , biology , in situ hybridization , microbiology and biotechnology , virology , gene , medicine , messenger rna , immunohistochemistry , biochemistry
To cast light on the significance of Epstein‐Barr virus (EBV) infection In cervical tumorigenesie, 44 cervical lntraepithellal neoplasia (CIN) types I/II, and 70 CIN III lesions, 60 lnvasive squamous cell carcinomas (ISCC), and 20 normal cervical samples were Investigated by polymerase chain reaction (PCR) and RNA in situ hybridization (RISH) assays. The EBV genome was detected by PCR using primers targetlng the IR region in three (6.8%0) of the CIN I/II, 15 (21.4%) of the CIN III and 13 (21.7%) of the ISCC lesions, while using an EBER oligonucieotide probe RISH revealed positive signals in Infiltrating lymphocytes located In the cervical stroma, but not in dysplastic or tumor cells. There was a significant correlation between the presence of EBV DNA and the degree of lymphoid cell infiltratlon ( P =0.0223). In contrast, none of the normal cervical samples that were without inflammation demonstrated any EBV infection. Thus, the results indicated that a positive result for EBV on PCR may be simply dependent on the amount of lymphocytes in cervical stroma, suggesting that this virus does not play a major role In the etiology of cervical neoplasia.