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Malignant lymphoma induction of rabbits with oral spray of Epstein‐Barr virus‐related herpesvirus from Si‐IIA cells (HTLV‐II‐transformed Cynomolgus cell line): A possible animal model for Epstein‐Barr virus infection and subsequent virus‐related tumors in humans
Author(s) -
Koirala Tirtha Raj,
Hayashi Kazuhiko,
Chen HongLi,
Ino Hideo,
Kariya Naoyuki,
Yanai Hiroyuki,
Choudhury Chitta Ranjan,
Akagi Tadaatsu
Publication year - 1997
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1997.tb04522.x
Subject(s) - virus , virology , epstein–barr virus , lymphoma , biology , antigen , antibody , herpesviridae , immunology , viral disease
Malignant lymphoma (ML) was Induced in eight of nine rabbb inoculated by oral spray of the cell‐free pellets from SI‐IIA culture (MLV‐ll‐transformed leukocyte cell line of the Cynomolgus‐producing Epsteln‐Barr virus (EBV)‐related herpesvirus) after 64–141 days. None of the rabbits inoculated with EBV from B‐95–8 cells or HTLV‐II from MOT cells developed ML. Malignant lymphomas were usually of diffuse, large‐cell or mixed type. HTLV‐II infection was excluded by the polymerase chain reaction (PCR) and the particle agglutination test. EBV‐encoded RNA‐1 and EBV‐related DNA were detected in the tumor tissues by In situ hybridization and PCR, respectively. Anti‐viral capsid antigen of EBV antibody (anti‐VCA) was observed 3 weeks after oral inoculation of Si‐IIA cell‐free pellets. Polymerase chain reaction revealed continuous detection of EBV‐related virus DNA in the peripheral blood leukocytes from 3 days after oral inoculation. These results show that ML induced orally wtth SI‐IIA cell‐free pellets was caused by EBV‐related herpesvirus harbored by Si‐IIA cells. Oral spray of EBV from B‐95–8 also induced EBV Infection in rabbits, which was confirmed both by the presence of anti‐VCA and by PCR. These oral infection and mallgnant lymphoma induction systems of rabbit using EBV‐related virus from Si‐IIA or human EBV are useful animal models for the study of EBV infection and EBV‐related lymphomas in humans.