Premium
Subcellular organelle analysis applying confocal laser scanning microscopy to the steroid‐producing epithelial ovarian tumor: A technical note
Author(s) -
Yasuda Masanori,
Itoh Johbu,
Muramatsu Toshinari,
Shinozuka Takao,
Sasano Hironobu,
Tsutsumi Yutaka,
Osamura R. Yoshiyuki
Publication year - 1997
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1997.tb04505.x
Subject(s) - endoplasmic reticulum , organelle , confocal , confocal laser scanning microscopy , pathology , stromal cell , subcellular localization , microbiology and biotechnology , biology , chemistry , medicine , biophysics , geometry , mathematics , cytoplasm
An epithelial ovarian tumor with steroid production was examined in a 70‐year‐old postmenopausal female. The stromal cells of this tumor were rather dense and occasionally characterized by tuteinization or hyperthecosis, which has been associated with steroidogenesis. Subcellular visualization using confocal laser scanning microscopy (CLSM) successfully led to the identification of 3β‐hydroxysteroid dehydrogenase (HSD) in both mitochondria‐like small particles and endoplasmic reticulum‐like linear profiles, and P450‐aromatase also in endoplasmic reticulum‐like linear profiles, on the three‐dimensional images.