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Helicobacter pylori infection in gastric xanthomas: lmmunohistochemical analysis of 145 lesions
Author(s) -
Hori Sadaaki,
Tsutsumi Yutaka
Publication year - 1996
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1996.tb03658.x
Subject(s) - pathology , immunoperoxidase , xanthoma , antigen , lamina propria , monoclonal antibody , antibody , biology , medicine , immunology , epithelium
A total of 145 paraffin‐embedded biopsy samples of gastric xanthoma were analyzed for the localization of Hellcobacter pylorl (HP) antigens. By the indirect immunoperoxidase method using a polyclonal antibody, HP lnfection was identffied on the surface of foveolar cells In 69 (48%) samples. In 38 (55%) of the 69 lesions, the HP antigens were demonstrated in the cytoplasm of xanthoma cells clustered in the actively inflamed lamina propria mucosae, Among the remalning 76 xanthoma lesions negative for HP lnfection on the epithellal surface, only eight (11%) showed the existence of HP antigens in the foamy histiacytes, and 39 (51 %) revealed mild inflammatory change. Monoclonal antibody study using 75 specimens also gave a comparable result. Pre‐embedding lmmunoelectron microscopy using paraffin sections revealed positively labeled rod‐shaped bacteria both on the epithelial surface and in the phagosome of the xanthoma cells. These findings strongly suggest that some of the xanthoma lesions are provoked by lamina proprial invasion of surface‐infected HP.