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Phenotypic modulation in cisplatin‐resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma
Author(s) -
Yamate Jyoji,
Tajima Masanorl,
Shibuya Kazumoto,
Kuwamura Mitsuru,
Kotani Takao,
Sakuma Sadashige
Publication year - 1996
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1996.tb03654.x
Subject(s) - histogenesis , myofibroblast , pathology , phenotype , biology , osteosarcoma , in vitro , histology , cisplatin , in vivo , immunohistochemistry , microbiology and biotechnology , cancer research , medicine , fibrosis , chemotherapy , gene , biochemistry , genetics
The histogenesis of malignant fibrous hlstlocytoma (MFH) was studled using clsplatln (CDDP)‐resistant MT‐R8 and MT‐R9 cells derlved from cloned undlfferentiated MT‐8 and flbrohlstlocytic MT‐9 cells, resoecthfely, which had been established from transplantable rat MFH. CDDP concentrations requlred for 50% suppression of prollferation of MT‐R8 and MT‐R9 cells were 5.4– and 3.3‐fold greater than those of parental MT‐8 and MT‐9, respectively. MT‐R8 and MT‐Rg showed the higher positive rates to histimytic lysosomal/ antigenic (ED1 and ED2) markers. The number of a‐smoath muscle actin (SMA)‐positive cells significantly Increased in MT‐RB; SMA‐positlve cells were also obsenred in MT‐R9, but no difference was seen between MT‐9 and MT‐R9. MT‐R8 and MT‐R9 expressed both histiwytic and myofibroblastic phenotypes. However, the histology of subcutaneous tumors induced in syngeneic rats by MT‐R8 and MR‐R9 did not always reflect their in vitro nature. MT‐R8 developed undiffer‐entlated sarcomas similar to parental MT‐8 tumors. In contrast, MT‐R9 induced tumors with polytypic histologies such as the storiform growth pattern, neoplastlc growth of granular cells and myofibroblasts, osteosarcoma‐like areas, collagen‐rich areas containing well‐developed fibroblasts and areas involvlng many lipoblasts. These In vivo observatfons suggest the multidlrectional differentiation of MT‐R9 cells. Phenotypic modulation of rat MFH cells seemed to be easily induced by CDDP. A possible histogenesis of MFH was discussed based on the data collected.