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Expression of classical protein kinase C subspecies in non‐neoplastic lymphocytes and non‐Hodgkin's lymphomas: An immunohistochemical study
Author(s) -
Hojo Hiroshi,
Morimura Yutaka,
Abe Masafumi,
Wakasa Haruki
Publication year - 1996
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1996.tb03591.x
Subject(s) - germinal center , mantle zone , lymphoma , pathology , immunohistochemistry , biology , working formulation , b cell , medicine , cancer research , non hodgkin's lymphoma , antibody , immunology
It is generally accepted that phospnorylation plays a pivotal role in the cellular response of cell differentiation and proliferation. Immunohistochemical expression of classical protein kinase C (cPKC) subspecies (α, β and γ) in eight reactive tymphold tissues, three normal spleens and 149 non‐Hodgkin's lymphomas was examined. cPKC β was observed primarily in the mantle zone B cells, but appeared as very faint staining In Ki‐67 positive proliferated B cells in the germinal centers of secondary lymph follicies. In contrast to the reactive state, high levels of cPKC subspecies were recognized in the majority of 149 cases of non‐Hodgkin's lymphoma, Including those thought to have arisen from germinal center cells such as follicular lymphoma. The expression of cPKC α was found in higher frequency in T cell lymphomas than B cell lymphomas ( P < 0.01) by the Chi‐squared test. High levels of cPKC α were present only in high grade or highly aggressive lymphomas, showing the highest incidence in the small non‐cleaved cell type, according to the International Working Formulation and National Cancer Institute ( P < 0.01). cPKC γ was not detected in normal lymphold cells and was expressed in only four cases of non‐Hodgkin's lymphomas. It is presumed that cPKC α and β have a relationship to cell activation and proliferation of lymphoid cells of reactive and neoplastlc states. It might be considered that the expression of cPKC α may have a relationship with aggressiveness in non‐Hodgkin's lymphomas.