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Identification of the cell type origin of odontoma‐like cell masses in microphthalmic ( mi/mi ) mice by in situ hybridization
Author(s) -
Nakajima Yuko,
Shimokawa Hitoyata,
Terai Kunihiro,
Onoue Hitoshi,
Seino Yoshiki,
Tanaka Hiroyuki,
Sobue Shizuo,
Kitamura Yukihiko,
Nomura Shintaro
Publication year - 1996
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1996.tb03543.x
Subject(s) - odontoblast , ameloblast , osteocalcin , osteopontin , in situ hybridization , pathology , ossification , columnar cell , epithelium , histogenesis , chemistry , biology , anatomy , messenger rna , microbiology and biotechnology , immunohistochemistry , medicine , endocrinology , pulp (tooth) , alkaline phosphatase , gene , dentistry , biochemistry , enamel paint , enzyme
Tooth abnormalities occur in microphthalmic ( mi/mi ) mice. The elongated odontogenlc epithelium is interrupted by un‐resorbed bone at the basal end of the mi/mi Incisor, with the epithelium gathered into cell clusters. These clusters develop to odontoma‐like masses. To identify the origin of the cell types of these odontoma‐like masses, the localization of osteonectin (Osn), osteocalcin (Osc), osteopontin (Osp), matrix Gla protein (MGP) and amelogenin (Am) mRNA in the process of tooth development in mi/mi and +/+ mice was investigated by means of in situ hybridization. Decalcified mandibles of neonatal, 5‐, 10‐, 14‐day‐old mice were examined. Osn and Osc mRNA, which localized in osteoblasts and odontoblasts, were also detected in the cells of odontoma‐like masses in mi/mi mice. The cells expressing these mRNA were short, columnar and odontoblast‐like. Am mRNA was detected In ameloblasts. In mi/mi mice, Am mRNA was also detected in ameloblastic cell clusters, which were formed by the tall columnar cells in the odontoma‐like masses. No apparent Osp mRNA expression was detected in the masses. These results indicated that even In odontogenlc abnormal cells resulting from physical obstruction in mi/mi mice, the genes that are involved in normal tooth development were still expressed.