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Integrin distribution in gastric carcinoma: Association of β3 and β5 integrins with tumor invasiveness
Author(s) -
Kawahar Ei,
Ooi Akishi,
Nakanishi Isao
Publication year - 1995
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1995.tb03491.x
Subject(s) - integrin , pathology , medicine , gastric carcinoma , cancer research , cancer , receptor
Immunolocalization of a variety of integrins using monoclonal antibodies against β3, β5, α3β1 and α6, and polyclonal antibodies against vitronectin receptor (αvβ3) and l were investigated on PLP‐fixed paraffin sections of 19 cases of advanced gastric carcinomas. The β5 integrin, which pairs only with the av subunit, was positive in seven cases (37%), and was associated closely with scirrhous invasion (P < 0.05). β5 was positive chiefly in the cytoplasm of carcinoma cells and infrequently in cell membranes. β3, which is another subunit pairing with av, was positive in six cases (32%), and tended to be associated with scirrhous invasion (P < 0.1). β3 was also located chiefly in the cytoplasm. Five of the seven β5‐positive cases showed coexpression of β3. Polyclonal antibodies to αvβ3 also showed a significant difference among the amounts of stroma (P < 0.05). Anti‐β1 antibodies showed clear positivity in many cases (89%). Of the β1 integrins, α3β1 was positive in a few cases (26%) without any preferential pattern, and laminin receptor subunit α6 stained on cell membranes of neoplastic epithelia in many cases of carcinoma (89%) except for mucinous carcinoma. These distinctive patterns of integrin positivity indicate a close association of β5 and β3 expression with scirrhous invasion in gastric carcinoma.