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Transient expression of type I collagen in glomeruli with anti‐Thy‐1 antibody‐induced mesangial proliferative lesions
Author(s) -
Yagi Mikio,
Yamamoto Tadashi,
Nagano Nobuo,
Kato Shinichiro,
Kusaka Masaru,
Kawasaki Katsutoshi,
Yaoita Eishin,
Kihara Itaru
Publication year - 1995
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1995.tb03477.x
Subject(s) - mesangial proliferative glomerulonephritis , glomerulonephritis , fibronectin , messenger rna , extracellular matrix , glomerulosclerosis , endocrinology , type iv collagen , lesion , immunohistochemistry , medicine , extracellular , pathology , renal glomerulus , antibody , chemistry , biology , kidney , immunology , laminin , microbiology and biotechnology , proteinuria , gene , biochemistry
Glomerular expression of extracellular matrices at the protein and mRNA levels was examined in rats with mesangial proliferative glomerulonephritis induced by the intravenous administration of a monoclonal anti‐rat Thy‐1 antibody. In close association with the mesangial proliferative lesion, type I collagen was imrnunostained at day 8 but not demonstrated at day 28 in the glomeruli of the kidneys. Type I collagen mRNA expression prominently increased in the nephritic glomeruli at day 4, prior to the appearance of type I collagen protein. In addition, fibronectin expression was also elevated in the diseased glomeruli at both the protein and mRNA levels. These results indicated that glomerular, probably mesangial cells, change their phenotypes during this disease, to synthesize abnormal extracellular matrices that lead to the progression of glomerular sclerosis.