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Interphase cytogenetics of gastric carcinoma: Fluorescence in situ hybridization (FISH) applied to cells obtained from formalin‐fixed paraffin‐embedded tissues
Author(s) -
Gomyo Yoshihito,
Andachi Hironobu,
Nagao Katsuhito,
Ikeguchi Masahide,
Ito Hisao
Publication year - 1995
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1995.tb03446.x
Subject(s) - biology , pathology , fluorescence in situ hybridization , aneuploidy , trisomy , karyotype , microbiology and biotechnology , chromosome , cytogenetics , medicine , genetics , gene
The interphase cytogenetics in formalin‐fixed and paraffin‐embedded gastric cancer tissues were examined by fluorescence in sku hybridization (FISH) with α‐satellite DNA probes. Two gastric carcinoma cell lines, TMK‐1 and MKN‐28, were first analyzed cytogenetically. Of 25 TMK‐1 cell karyotypes, chromosome 7 showed trisomy and chromosome 17 showed disomy in 18 cells. Most MKN‐28 cells showed disomy of both chromosomes 7 and 17. Suspensions of singly isolated TMK‐1 and MKN‐7 cells were obtained from the cultured cells, and from paraffinembedded tissue specimens fixed with formalin for 0, 1, 3 and 5 days obtained from xenotrans‐planted tumors in nude mice. The numbers of chromosomes 7 and 17 analyzed with the karyotypic preparations coincided well with those determined by FISH, even in the paraffin‐embedded specimens. The number of tumor cells showing no signals, however, increased in the specimens after 5 days formalin fixation. In 10 surgically removed gastric carcinomas, the predominant signal number for chromosomes 7 and 17 in the cells of paraffinembedded tissues was two (disomy), except in one papillary carcinoma, which was trisomic for chromosome 7. Large subpopulations (more than 20%) showing trisomy were found in four cases for chromosome 7 and in five cases for chromosome 17. A higher frequency of trisomy was found in well differentiated than in poorly differentiated carcinomas. These findings suggest that the FISH technique is a useful tool for detecting chromosomal aberrations in gastric adenocarcinoma cells, even in paraffinembedded specimens, as long as the tissues are fixed with formalin for an appropriate time.