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Establishment of a human hepatic adenosquamous carcinoma cell line (KMC‐2) and its response to cytokines
Author(s) -
Momosaki Seiya,
Yano Hirohisa,
Kojiro Masamichi
Publication year - 1995
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1995.tb03434.x
Subject(s) - doubling time , adenocarcinoma , adenosquamous carcinoma , population , cell culture , antigen , transplantation , pathology , biology , carcinoma , nude mouse , cancer research , secretion , cancer , immunology , medicine , endocrinology , genetics , environmental health
A human hepatic adenosquamous carcinoma cell line (KMC‐2) was established from a serially transplanted tumor in nude mice (nuKMC‐2), which originated from human cholangio‐cellular carcinoma and showed histological alteration from adenocarcinoma to squamous cell carcinoma (SCC) along with serial transplantation. KMC‐2 cells in monolayer culture proliferated in a sheet‐like arrangement with a population doubling time of 29.5 h, whereas the cells in 0.1% collagen gel embedded culture formed a compact and tubular structure with the population doubling time of 35.4 h. The cells secreted carbohydrate antigen 19–9 (CA19–9), tissue polypeptide antigen and SCC‐related antigen. The back‐transplanted nude mouse tumor exhibited morphologic features of adenosquamous carcinoma resembling those in the original nude mouse tumor. IFN‐α, IFN‐γ and TNF‐α suppressed cell proliferation significantly. Functionally, IFN‐γ significantly suppressed CA19–9 secretion, and conversely promoted SCC‐related antigen secretion. These findings suggest that KMC‐2 is the first human hepatic adenosquamous carcinoma cell line primarily originated from adenocarcinoma; the environmental factors, such as the presence of extracellular matrix and the cytokines influenced the growth, morphology and function of KMC‐2.