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Morphological aspects of LFA‐1/ICAM‐1 and VLA4/VCAM‐1 adhesion pathways in human lymph nodes
Author(s) -
Tanaka Hisako,
Saito Saburo,
Sasaki Hiroyuki,
Arai Hisako,
Oki Takao,
Shioya Naoshi
Publication year - 1994
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1994.tb03364.x
Subject(s) - lymphocyte homing receptor , vcam 1 , high endothelial venules , cell adhesion molecule , lymph , icam 1 , venule , addressin , intercellular adhesion molecule 1 , endothelium , intercellular adhesion molecule , microbiology and biotechnology , lymphocyte function associated antigen 1 , lymphocyte , biology , cell adhesion , pathology , chemistry , adhesion , immunology , medicine , endocrinology , organic chemistry
Monoclonal antibodies specific for the adhesion molecules participating in lymphocyte homing, lymphocyte function associated antigen‐1 (LFA‐1) and very late antigen 4 (VLA4), and their respective ligands, intercellular adhesion molecule 1 (ICAM‐1) and vascular cell adhesion molecule 1 (VCAM‐1), were used to characterize their expression pattern in human lymph nodes by immunohistochemical and immunoelectron microscopic techniques. The location of LFA‐1‐positive lymphocytes and selective expression of ICAM‐1 on the luminal plasma membrane of high endothelial venule endothelium suggested that the LFA‐1/ICAM‐1 adhesion pathway participates only in the initial step of the lymphocyte migration process. Lymphocytes passing through endothelium appear not to be influenced by this pathway. VCAM‐1 was detected occasionally on the endothelium of high endothelial venules in the hyperplastic lymph nodes in the mesentery, but not in peripheral lymph nodes. VLA4‐positive lymphocytes tended to be more frequently observed within high endothelial venules in mesenteric lymph nodes than in peripheral ones. Strong expression of both ligands, ICAM‐1 and VCAM‐1, was noted on the plasma membrane of follicular dendritic cells, and was especially prominent on their labyrinthine folding, and on the interdigitating cells in the paracortex. Furthermore, both LFA‐1‐and VLA4‐positive lymphocytes localized around these cells. This suggests that LFA‐1/ICAM‐1 and VLA4/VCAM‐1 adhesion pathways play an important role in the lymphocyte recognition of antigen‐presenting cells.