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Effect of traditional Chinese medicine (Sairei‐to) on monoclonal antibody‐induced proteinuria in rats
Author(s) -
Kawachi Hiroshi,
Takashima Noriko,
Orikasa Michiaki,
Oite Takashi,
Shimizu Fujio
Publication year - 1994
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1994.tb02933.x
Subject(s) - proteinuria , medicine , urology , monoclonal antibody , renal function , endocrinology , antibody , kidney , immunology
The effects of traditional Chinese medicine (Sairei‐to) on monoclonal antibody (mAb) inducing proteinuria were examined. Four hundred, 200 and 100mglkg body weight (BW) of Sairei‐to and phosphate‐buffered saline (PBS) as a control were injected intraperitoneally into four groups of female Wistar rats every day from 5 days before intravenous injection of mAb to the end of the experimental period. The amount of urinary protein excretion was significantly suppressed in roughly a dose‐dependent manner. For example, 116.6 ± 89.7mg/day of proteinuria was observed in control groups compared to 4.2 ± 15.2 mglday in the 400 mglkg BW of Sairei‐to treated group 2 days after mAb injection (P < 0.01). Statistically significant (P <0.01) differences were again observed in a repeat experiment (122.1 ± 53.7 vs 10.2 ± 10.1 mglday on the 2nd day) without affecting the glomerular filtration rate. No significant difference was recognized between the total amount of mAb bound to glomeruli 1 h after mAb injection in Sairei‐to‐treated and non‐treated rats, indicating that Sairei‐to pretreatment has no effects on the number or quality of antigenic molecules. The effect of Sairei‐to on a non‐immunological model of proteinuria was also examined. No significant reduction of proteinuria by similar Sairei‐to treatment was observed in aminonucleoside of puromycin nephropathy. The authors conclude that mAb‐induced proteinuria in rats is significantly suppressed by the traditional Chinese medicine, Sairei‐to.