Open AccessRe‐evaluation of a case of progressive multifocal leukoencephalopathy previously diagnosed as simian virus 40 (SV40) etiology
Author(s) -
Eizuru Yoshito,
Sakihama Kuniharu,
Minamishima Yoichi,
Hayashi Tohru,
Sumiyoshi Akinobu
Publication year - 1993
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1993.tb02575.x
Subject(s) - ecori , bamhi , virology , progressive multifocal leukoencephalopathy , virus , biology , dna , restriction enzyme , jc virus , microbiology and biotechnology , hindiii , simian , genetics
A case of progressive multifocal leukoencephalopathy (PML) reported previously to be of simian virus (SV40) etiology was re‐evaluated. The supernatant from a 10% homogenate of brain material was inoculated into African green monkey kidney cells and BSC‐1 cells which are permissive for SV40. However no cytopathic effect (CPE) developed and no virus was isolated. The brain supernatant agglutinated human group O erythrocytes and contained 5120 units/mL. The Hirt supernatant from the brain contained three DNA bands corresponding to forms I, II and III of circular double‐stranded viral DNA. Restriction endonuclease cleavage analysis revealed that this viral DNA was different from SV40 DNA, but similar to JC virus DNA. After cloning of this viral DNA into pBR322 at the Bam HI site, DNA homology of this virus and of SV40 was investigated. The cloned DNA hybridized with all four Hpa I/ Eco RI fragments of SV40 genome at the effective temperature (Tm) of −50°C. At Tm −28°C, however, the cloned DNA hybridized with only Hpa I/ Eco RI fragment B of the SV40 genome. In contrast to this, JC virus DNA hybridized with all five Eco RI/ Bam HI/ Hind III fragments of cloned DNA even at Tm −28°C. Therefore, the causative agent of this PML case was not SV40 but JC virus.