Open AccessExpression Pattern of SRα Promoter in Human Embryonal Carcinoma and Transgenic Tissues in Mice
Author(s) -
Fujita Hiroo,
Hata Junichi,
Kokai Yasuo,
Matsubayashi Yoshie,
Takabe Yutaka,
Fujimoto Junichiro
Publication year - 1992
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1992.tb03220.x
Subject(s) - embryonal carcinoma , biology , genetically modified mouse , transgene , microbiology and biotechnology , gene , carcinoma , cell culture , gene expression , fusion gene , cancer research , cellular differentiation , genetics
Human embryonal carcinoma is thought to be a counterpart of mouse embryonal carcinoma, which has provided useful information for studying early molecular events in murine embryogenesis. A major practical problem in the use of human embryonal carcinoma for molecular pathological studies is the lack of an efficient expression system for foreign genes. The SRα promoter is a fusion promoter containing the SV 40 early promoter and the R segment and part of the U5 sequence of the long terminal repeat derived from human T cell leukemia virus type I. We analyzed the expression pattern of the SRα promoter in human and mouse embryonal carcinoma lines and transgenic mouse tissues. Efficient and stable expression was detected in all cell lines tested, and tissues from all mice of four independent transgenic lines carrying the SRα CAT vector showed a detectable level of CAT expression. These data suggest that the SRα promoter is useful for studies of both human embryonal carcinoma and transgenic mouse tissue. Using this expression system, we are now able to label human embryonal cacinomas with various genes, for example β galactosidase, and follow their fate at the single cell level in nude mice, where xenotransplanted human embryonal carcinoma expresses differentiation capability. Acta Pathol Jpn 42: 712–718, 1992.