Open AccessEffects of Alkyl Alcohols and Related Chemicals on Rat Liver Structure and Function: III. Physicochemical Properties of Ethanol‐, Propanol‐ and Butanol‐treated Rat Liver Mitochondrial Membranes
Author(s) -
Adachi Kayo,
Momota Michihiko,
Teranishi Yoshiki,
Ueki Reiko,
Hagiwara Masatoshi,
Wakabayashi Takashi,
Popinigis Jerzy
Publication year - 1992
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1992.tb03104.x
Subject(s) - chemistry , phosphatidylinositol , phosphatidylethanolamine , membrane fluidity , membrane , phosphatidylcholine , butanol , propanol , fluorescence anisotropy , diphenylhexatriene , ethanol , chromatography , phospholipid , biophysics , biochemistry , biology , kinase
The physicochemical properties of mitochondria in liver tissue obtained from rats given 32% ethanol, 32% propanol or 6.9% butanol in drinking water for up to 3 months were investigated using differential scanning calo‐rimetry and fluorescence polarization measurements. The results obtained were as follows: 1) Phospholipids extracted from mitochondria showed increases in the relative amounts of phosphatidylcholine, phosphatidylinositol and phosphatidylserine, and a decrease in the relative amount of phosphatidylethanolamine. An increase in the unsatu‐rated/saturated fatty acid ratio of phospholipids was also observed. 2) Elevation of the thermotropic lipid phase transition temperature with a decrease in the enthalpy value (δ H ) was revealed by differential scanning calo‐rimetry. 3) The elevation of the lipid phase transition temperature was detected also by fluorescence polarization measurements using 1,6 diphenyl 1, 3, 5 hexatriene (DPH) as a probe. Elevation of mitochondrial membrane fluidity was found in some of the experimental animals, but most showed no changes in comparison with the control. A possible role of membrane fusion in the mechanism of formation of ethanol‐, propanol‐ and butanol induced hepatic megamitochondria is discussed on the basis of these results. Acta Pathol Jpn 42: 549–557, 1992.