Open AccessDendritic Cells in the Murine Dermis in Delayed‐type Contact Hypersensitivity
Author(s) -
Kuwabara Hiroko,
Uda Hirotsugu,
Saito Katsuhiro,
Maruyama Toshiyuki,
Tanaka Sumiko
Publication year - 1991
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1991.tb02505.x
Subject(s) - dermis , pathology , mononuclear phagocyte system , monoclonal antibody , immunohistochemistry , dendritic cell , peripheral blood mononuclear cell , antigen presenting cell , langerhans cell , biology , antibody , antigen , medicine , t cell , immunology , in vitro , immune system , biochemistry
Dendritic cells within the dermis in the later stages of delayed‐type contact hypersensitivity were examined ultra‐structurally and immunohistochemically. The immunohis‐tochemical observations were done using monoclonal antibody Ml 8, which reacts specifically with murine Langer‐hans cells and interdigitating cells. Seventeen hours after challenge, infiltrating cells in the dermis included dendritic cells, possibly so‐called indeterminate cells, monocytoid cells and Langerhans cells. Immunohistochemically, the indeterminate cells and some monocytoid cells were Ml 8 positive. These findings suggest that indeterminate cells are intimately related to Langerhans cells, and that they belong to the mononuclear phagocyte system. Ml‐8 is a very useful marker for studies on the kinetics of Langerhans cells or indeterminate cells. Acta Pathol Jpn 41: 106 112, 1991.