Open AccessImmunohistochemicaI and Imrnunoblotting Detection of Cytokeratin in Smooth Muscle Tumors
Author(s) -
Tauchi Katsunori,
Tsutsumi Yutaka,
Yoshimura Shinichi,
Watanabe Keiichi
Publication year - 1990
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1990.tb01602.x
Subject(s) - cytokeratin , pathology , leiomyosarcoma , smooth muscle tumor , immunohistochemistry , monoclonal antibody , smooth muscle tissue , biology , vimentin , intermediate filament , smooth muscle , antibody , medicine , cytoskeleton , cell , immunology , endocrinology , genetics
Forty six smooth muscle tumors, including 35 of gastrointestinal origin, were studied immunohistochemically for the localization of cytokeratin using a variety of monoclonal antibodies. In formalin‐fixed, paraffin embedded material, six of 40 leiomyomas, two of five leiomyosar‐comas and one leiomyoblastoma were immunoreactive for cytokeratin in a few tumor cells. A proportion of non neoplastic smooth muscle cells also exhibited a positive reaction. In fresh frozen sections of one gastric leio‐myosarcoma, a high percentage of tumor cells were reactive with nine of the eleven anti cytokeratin monoclonal antibodies examined. Cytokeratin positive cyto‐plasmic filaments were further demonstrated by im‐munoelectron microscopy. By immunoblot analysis, an extract of this immunohistochemically cytokeratin‐positive leiomyosarcoma showed a distinct band at the same position as an extract of pancreas, whereas no bands were seen in an extract of a cytokeratin‐negative esophageal leiomyosarcoma. These immunohistochemical and immunoblotting findings indicate that a certain subset of smooth muscle neoplasms express genuine cytokeratin filaments.