Open AccessInhibition of Pancreatic Hepatocyte Induction in Hamsters Treated Sequentially with Ethionine, a Protein‐free Diet and then Methionine by Prior N‐nitrosobis‐(2‐oxopropyI)amine Administration
Author(s) -
Kitazawa Shunji,
Mizumoto Kazuhiro,
Amanuma Toshihiro,
Tsutsumi Masahiro,
Maruyama Hiroshi,
Konishi Yoichi
Publication year - 1990
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1990.tb01600.x
Subject(s) - ethionine , methionine , administration (probate law) , medicine , endocrinology , chemistry , hepatocyte , biochemistry , amino acid , political science , law , in vitro
A 100% yield of pancreatic hepatocytes was induced in pancreas tissues of female hamsters treated with twice‐repeated sequential administrations of DL‐ethionine (ethionine) together with a protein‐free diet and then L‐methionine (methionine) for 10 weeks. The cells were also found in 40% of hamsters receiving 20mg/kg body weight of the pancreatic carcinogen, N nitrosobis(2‐oxopropyl)amine (BOP) given twice at the peak of pancreatic regeneration stimulated by methionine after ethionine induced cell damage. However, BOP at doses of 30, 70, and 100 mg/kg body weight administered before the occurrence of pancreatic regeneration dose dependently inhibited their appearance, with reduction of the yield to 40%, 25%, and 8.3% respectively, and BOP per se did not induce any development of pancreatic hepatocytes. Stein iodine staining revealed bile pigments in the induced hamster pancreatic eosinophilic cell populations.