Open AccessSequential Analysis of Distributions of Donor‐derived Thymocytes Bearing T‐cell Antigen Receptor (TCR) and Donor‐derived la + Cells in Thymuses of Fully Allogeneic Bone Marrow Chimera in Mice
Author(s) -
Hatakeyama Shigetsugu,
Ogasawara Kazumasa,
Fukushi Noriko,
Iwabuchi Chikako,
Iwabuchi Kazuya,
Wang Bingyan,
Kajiwara Masaharu,
Good Robert A.,
Onoè Kazunori
Publication year - 1990
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1990.tb01578.x
Subject(s) - chimera (genetics) , medulla , t cell receptor , bone marrow , thymocyte , biology , antigen , receptor , microbiology and biotechnology , chemistry , pathology , immunology , t cell , endocrinology , cd8 , medicine , immune system , gene , biochemistry
Lethally irradiated SJL/J mice were reconstituted with B10 bone marrow cells, and the process of thymic reconstitution by donor derived cells positive for I‐ A or Vβ8 molecules was investigated. The donor‐derived la + cells appeared in the medulla on day 7 after reconstitution. The la+ cells became confluent up to day 14, and the cellularity in the medulla on day 17 was almost the same as that in the normal thymus. Dull Vβ8 + thymocytes were first recognized in the cortex on day 10 and were identifiable in the medulla by day 14. The Vβ8 + cells seemed to be mainly CD4 + 8 + double‐positive. Furthermore, most of the Vg8’cells in the medulla of chimeras given cyclosporin A for 3 weeks after reconstitution appeared to be CD4 + 8 + . The present findings demonstrate that CD4 8+ thymocytes which bear a low concentration of TCR exist in the thymic medulla at a relatively early stage when donor‐derived la + cells have already settled there. The coincidental appearance and coexistence of la + cells and TCR + thymocytes in the medulla suggest that these histological characteristics may be related to the selection of thymocytes in this area.