Open AccessIn Situ Localization of Pepsinogens I and II mRNA in Human Gastric Mucosa
Author(s) -
Sano Junjiro,
Miki Kazumasa,
Ichinose Masao,
Kimura Masayoshi,
Kurokawa Kiyoshi,
Aida Takeo,
Ishizaki Masamichi,
Asano Goro,
Masugi Yozo,
Wong Ricky N.S.,
Takahashi Kenji
Publication year - 1989
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1989.tb02428.x
Subject(s) - gastric mucosa , in situ hybridization , pathology , in situ , messenger rna , biology , medicine , stomach , chemistry , gene , genetics , organic chemistry
Localization of pepsinogens I and II mRNA in the human gastric mucosa was investigated by an in situ hybridization method using digoxigenin labeled cDNA probes. Gastric fundic mucosa from healthy volunteers, which was stained with digoxigenin labeled pepsinogens I and II cDNA probes, showed positive staining in the cytoplasm of both chief cells and mucous neck cells. In contrast, gastric antral mucosa stained with the pepsinogen I cDNA probe showed no positive reaction in the surface mucous cells or pyloric glands. On the other hand, the pyloric glands were stained positively with the pepsinogen II cDNA probe and the staining appeared to be identical to that obtained with the anti pepsinogens I and II monoclonal antibodies using the avidin biotin peroxidase complex technique. These results are consistent with those of previous studies that have employed immunochemical and immunohistochemical techniques. Acta Pathol Jpn 39: 765 771, 1989.