Open AccessLocalization of Type III Procollagen mRNA in Areas of Liver Fibrosis by in situ Hybridization
Author(s) -
Yamada Hiroyuki,
Aida Takeo,
Taguchi Katsuji,
Asano Goro
Publication year - 1989
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1989.tb02420.x
Subject(s) - in situ hybridization , procollagen peptidase , pathology , messenger rna , in situ , fibrosis , microbiology and biotechnology , medicine , biology , chemistry , biochemistry , gene , organic chemistry
Localization of type III procollagen mRNA in human liver was studied by in situ hybridization using human 0‐1(111) procollagen cDNA. Frozen and paraformaldehyde‐fixed sections of biopsied human liver from patients with chronic hepatitis and cirrhosis were examined using the digox‐igenin labeled cDNA probe. Localization of the type III procollagen mRNA was demonstrated not only in the cytoplasm of mesenchymal cells but also in a large number of hepatocytes, in proportion to the extent of fibrosis. These results suggest that hepatocytes play an important role in fibrogenesis in the liver.