Open AccessHistochemical Analysis of Changes in Lectin Binding in Murine Glomerular Lesions
Author(s) -
Kizaki Tomohiko,
Takeda Zenju,
Watanabe Makoto,
Hanioka Keisuke,
Itoh Hiroshi
Publication year - 1989
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1989.tb02400.x
Subject(s) - wheat germ agglutinin , lectin , basement membrane , ulex europaeus , glomerular basement membrane , griffonia simplicifolia , peanut agglutinin , agglutinin , microbiology and biotechnology , concanavalin a , biology , pathology , soybean agglutinin , chemistry , biochemistry , anatomy , kidney , glomerulonephritis , endocrinology , medicine , in vitro
Lectin binding in diseased murine glomeruli was studied in MRL 1 mice, using seven different fluorescence‐ or peroxidase coupled lectins: Griffonia simplicifolia I (GS‐I) , Ulex europaeus agglutinin I (UEA‐I) , Ricinus communis agglutinin I (RCA I), wheat germ agglutinin (WGA), concanavalin A (Con A), peanut agglutinin (PNA), and Helix pomatia agglutinin (HPA). Lectin binding in diseased glomeruli of MRL 1 mice was different from that in normal glomeruli. Light and fluorescence microscopy showed that: 1. in mesangial proliferative lesions, the binding of RCA I, WGA and Con A increased and that of GS I and PNA appeared in the mesangium; 2. in other glomerular lesions, UEA‐I bindng appeared and RCA I stained the altered membranes irregularly. Electon microscopy showed that: 1. GS‐I stained the endothelial cell coat and the glomerular basement membrane covered by the endothelial cells; 2. GS I strongly stained the dilated subendothelium in regions of mild mesangial interposition; 3. GS‐I stained the cell coat of invasive macrophages; 4. GS‐I and UEA‐I stained the cell membrane‐like material derived from degenerative endothelial cells; 5. RCA I stained the epithelial and endothelial cell coats and the glomerular basement membrane. These results indicate that lectin‐binding studies can be used for analysis of glomerular lesions.