Open AccessEXPERIMENTAL IN VIVO REGENERATION OF PERIPHERAL NERVE AXONS and PERINEURIUM GUIDED BY RESORBABLE COLLAGEN FILM
Author(s) -
Takahashi Manabu,
Satou Takao,
Hashimoto Shigeo
Publication year - 1988
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1988.tb02289.x
Subject(s) - perineurium , regeneration (biology) , silicone , epineurium , matrix (chemical analysis) , anatomy , in vivo , peripheral nerve , fibrin , chemistry , medicine , microbiology and biotechnology , biology , organic chemistry , chromatography , immunology
In our previous paper, we reported a marked advantage in using collagen gel matrix available for cell culture in combination with a silicone tube as an effective environment for axonal sprouting during peripheral nerve regeneration. In the present experiment, collagen film was substituted for the silicone tube because of its non‐toxicity, biocompatibility and better availability. Also, the surgical procedure was simplified, using a fibrin adhesive system instead of suturing. In the second postoperative week, severed proximal and distal stumps became joined together concomitantly with absorption of the collagen matrix and film. On size‐frequency histograms, the diameters of both myelinated and unmyelinated axons at 8 weeks after surgery had recovered to their normal ranges. These findings demonstrate that this procedure of enveloping a collagen matrix and severed nerve stumps in bioresorbable collagen film would be an ideal way of forming a perfect perineurium. The regeneration of peripheral nerve axons resulted in normal thickness of the original nerve bundle without exception, unlike the axons on the control side.