Open AccessELECTRON MICROSCOPIC EVIDENCE FOR EPIDERMAL GROWTH FACTOR RECEPTOR (EGF‐R)‐LIKE IMMUNOREACTIVITY ASSOCIATED WITH THE BASOLATERAL SURFACE OF GASTRIC PARIETAL CELLS
Author(s) -
Mori Shigeo,
Morishita Yasuyuki,
Sakai Keisuke,
Kurimoto Shigeharu,
Okamoto Makoto,
Kawamoto Tomoyuki,
Kuroki Toshio
Publication year - 1987
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1987.tb03305.x
Subject(s) - parietal cell , epidermal growth factor , immunostaining , endoplasmic reticulum , gastric glands , biology , secretion , polyclonal antibodies , bone canaliculus , intracellular , foveolar cell , microbiology and biotechnology , receptor , chemistry , pathology , antibody , endocrinology , immunohistochemistry , gastric mucosa , stomach , anatomy , immunology , biochemistry , medicine , gastric acid
Epidermal growth factor (EGF), in addition to its effects on cell growth, has a suppressive effect on gastric hydrochloride secretion. We recently demonstrated EGF‐receptor (EGF‐R)‐like immunoreactlvity on human gastric parietal cells by light microscopy. To reveal further the localization of this reaction an immuno‐electron microscopical study was performed. 5281gG, an anti‐EGF‐R murine monoclonal antibody and a polyclonal anti‐EGF antibody were employed for immunostaining using the avidin‐biotin method. Positive reaction against 5281gG was shown mostly on the outer membrane of parietal cells, except in the apical portion. No reaction was observed on most of the intracytoplasmic membranes including intracellular canaliculi, endoplasmic reticulum and other membranous components. In the basillar portion, the 5281 gG‐positive parietal cell membrane formed labyrinthine interdigitations. No reaction against anti‐EGF was demonstrated in any of the gastric mucosal cells, although a reaction was clearly shown on salivary gland cells. These findings suggest a blood‐mediated direct action of EGF on parietal cells.