Open AccessPASSIVE TRANSFER OF EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS WITH LYMPHOCYTES COLLECTED FROM BRAINS OF IMMUNIZED GUINEA PIGS
Author(s) -
Ohkawa Tokitada,
Chino Fumitoshi
Publication year - 1987
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1987.tb00461.x
Subject(s) - encephalomyelitis , meninges , choroid plexus , pathology , guinea pig , medicine , spleen , spinal cord , pia mater , lymph node , lymph , immunology , parenchyma , peripheral blood mononuclear cell , central nervous system , multiple sclerosis , biology , biochemistry , psychiatry , in vitro
Lymphocytes were collected from the brains of guinea pigs immunized with syngeneic spinal cord emulsified in complete Freund's adjuvant. Sixteen to 32 times 10 6 lymphocytes were inoculated into the vein of each inbred guinea pig (NIH 13). Recipient animals without clinical signs of experimental allergic encephalomyelitis (EAE) were sacrificed 20 days later. Some of the recipients had perivascular infiltrates of a large number of mononuclear cells mostly in choroid plexus and meninges of the brain and spinal cord. Demyelination, which was not so intense, was also observed in the vicinity of the perivascular infiltrates. Thus, the lymphocytes from the brain as well as lymph node and spleen were clear to have the ability to transfer EAE.