Open AccessLOCALIZATION OF FERRITIN IN HUMAN LIVER DISEASES STUDIED BY IMMUNO‐HISTOCHEMICAL AND IMMUNO‐ELECTRON MICROSCOPIC PROCEDURES
Author(s) -
Ishimatsu Toshiyuki,
Nakayama Iwao
Publication year - 1987
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1987.tb00455.x
Subject(s) - ferritin , golgi apparatus , endoplasmic reticulum , staining , hemosiderin , pathology , cytosol , biology , intracellular , chemistry , microbiology and biotechnology , biochemistry , medicine , enzyme
Localization of ferritin using a pre‐embedding diffusion technique and an indirect localization sequence has been made in 34 cases of human liver under normal and several pathological conditions. With light microscopic observation, positive immuno‐staining for ferritin was demonstrated as diffuse deposits in the hepatocytes and Kupffer cells. Intensity of the positive immuno‐staining for ferritin in these cells appeared to roughly coincide with serum ferritin levels of each patient, but showed no disease specificity, although hepatoma cells contained weak deposits or were negative from immuno‐staining for ferritin. With electron microscopic studies, intracellular antigen was well preserved in the hepatocytes and Kupffer cells in most cases with the positive immuno‐staining for ferritin being observed in cytosol and a few cisternae of rough endoplasmic reticulum. Content of the positive immuno‐staining for ferritin differed considerably from one case to another and one cell to another even in the same case. There was no immuno‐staining for ferritin in hemosiderin pigment, lysosome, most of rough endoplasmic reticulum, Golgi complexes, and nucleus in both cells.